Not on the rev6 A500, mate. The hack is intended for rev6.x A2000 units.
I was after a suitable (A3000) Agnus to validate the hack for the rev6 A500 but the 8372AB Agnus chips I found was way too expensive to bought.
Of course you can solder another 512kb (4 chips) to the rev6 mobo and do a cut&join hack to have 1Mb chip RAM with no trapdoor card, as I did on two of mine rev6 A500.