Nope, I'm using it with a 3.1 install and a 3.5 install on a different hard drive. The 3.5 was already on the drive when I bought the Amiga tower so it's a bit of time capsule I am looking to preserve. I guess the 3.1 install is a Classic Workbench I've gradually customized over the years and I also have put this on a harddrive and not a cf card. OS3.2 doesn't require and IDEfix or 4xEIDE'99 driver as it recognises the two drives as far as I know.
I was thinking of connecting up the CD drive I have, but it is faulty as the door keeps opening by itself.